Skip to main content
Addgene

pMJA180
(Plasmid #128538)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128538 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pPIC6alpha
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3607
  • Vector type
    Yeast Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Blasticidin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    E coli BirA
  • Alt name
    DF192_21550
  • Species
    Escherichia coli
  • Insert Size (bp)
    967
  • Promoter AOX1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SacII (not destroyed)
  • 5′ sequencing primer CGACTGGTTCCAATTGACAAGCTTT
  • 3′ sequencing primer TGCATCTCTCAGGCAAATGGCA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

secreted E coli BirA a biotinylation synthetase protein in Pichia

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMJA180 was a gift from Marcos Alcocer (Addgene plasmid # 128538 ; http://n2t.net/addgene:128538 ; RRID:Addgene_128538)

  • For your References section:

    Production of in vivo biotinylated scFv specific to almond (Prunus dulcis) proteins by recombinant Pichia pastoris. de la Cruz S, Alcocer M, Madrid R, Garcia A, Martin R, Gonzalez I, Garcia T. J Biotechnol. 2016 Jun 10;227:112-9. doi: 10.1016/j.jbiotec.2016.04.024. Epub 2016 Apr 13. 10.1016/j.jbiotec.2016.04.024 PubMed 27085890
Related items:
Commonly requested with: