pMJA104
(Plasmid #128520)

• Purpose: Expresses amyloid human Lysozyme in Pichia pastoris
• Depositing Lab: Marcos Alcocer
• Publication: Johnson et al J Mol Biol. 2005 Sep 30;352(4):823-36. doi: 10.1016/j.jmb.2005.07.040.

Backbone

• Vector backbone: pPIC9
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 8005
• Vector type: Yeast Expression
• Selectable markers: HIS4

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Artificial gene for human lysozyme I56T
• Alt name: Lyz or X75362.1
• Species: H. sapiens (human)
• Insert Size (bp): 393
• Entrez Gene: LYZ (a.k.a. LYZF1, LZM)
• Promoter: AOX1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AvaI partial (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CGACTGGTTCCAATTGACAAGCTTT
• 3′ sequencing primer: TGCATCTCTCAGGCAAATGGCA

Resource Information

• Supplemental Documents:
  • pMJA104=Hu LysozymeI56T.gb
• A portion of this plasmid was derived from a plasmid made by: Lysozyme gene given by Prof Archer IFRN.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Secreted I56T amyloid Human mature lysozyme. Also reference PMID: 16441658.