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Addgene

pMJA034
(Plasmid #128514)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128514 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 5470
  • Vector type
    Bacterial Expression
  • Selectable markers
    Unspecified

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Xylanase A +uidA
  • Alt name
    AJ312295.1
  • Species
    Talaromyces funiculosus
  • Insert Size (bp)
    1479
  • Promoter Histone 4B

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site ClaI (not destroyed)
  • 5′ sequencing primer ATTACGCCAAGCTCGAAATTAACCCT
  • 3′ sequencing primer GTAAAACGACGGCCAGT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

expression of Xylanase A fused to GUS reporter gene

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMJA034 was a gift from Marcos Alcocer (Addgene plasmid # 128514 ; http://n2t.net/addgene:128514 ; RRID:Addgene_128514)

  • For your References section:

    Comparison of modular and non-modular xylanases as carrier proteins for the efficient secretion of heterologous proteins from Penicillium funiculosum. Alcocer MJ, Furniss CS, Kroon PA, Campbell M, Archer DB. Appl Microbiol Biotechnol. 2003 Feb;60(6):726-32. doi: 10.1007/s00253-002-1184-4. Epub 2002 Dec 21. 10.1007/s00253-002-1184-4 PubMed 12664153
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