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Addgene

pMJA008
(Plasmid #128512)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128512 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4205
  • Vector type
    Bacterial Expression
  • Selectable markers
    Unspecified

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Xylanase C
  • Alt name
    AJ278385.1
  • Species
    Talaromyces funiculosus
  • Insert Size (bp)
    727
  • Promoter Histone 4B

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer ATTACGCCAAGCTCGAAATTAACCCT
  • 3′ sequencing primer GTAAAACGACGGCCAGT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Use of histone promoter to drive expression of secreted Xylanase C in Penicillium funiculosum. Also reference 12466887.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMJA008 was a gift from Marcos Alcocer (Addgene plasmid # 128512 ; http://n2t.net/addgene:128512 ; RRID:Addgene_128512)

  • For your References section:

    A family 11 xylanase from Penicillium funiculosum is strongly inhibited by three wheat xylanase inhibitors. Furniss CS, Belshaw NJ, Alcocer MJ, Williamson G, Elliott GO, Gebruers K, Haigh NP, Fish NM, Kroon PA. Biochim Biophys Acta. 2002 Jul 29;1598(1-2):24-9. PubMed 12147340
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