pMJA008
(Plasmid #128512)

• Purpose: Expresses Xylanase C
• Depositing Lab: Marcos Alcocer
• Publication: Furniss et al Biochim Biophys Acta. 2002 Jul 29;1598(1-2):24-9.

Backbone

• Vector backbone: pBluescript
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 4205
• Vector type: Bacterial Expression
• Selectable markers: Unspecified

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Xylanase C
• Alt name: AJ278385.1
• Species: Talaromyces funiculosus
• Insert Size (bp): 727
• Promoter: Histone 4B

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: ATTACGCCAAGCTCGAAATTAACCCT
• 3′ sequencing primer: GTAAAACGACGGCCAGT

Resource Information

• Supplemental Documents:
  • pMJA008= His4prom+XynC+XynCTerm.gb
• A portion of this plasmid was derived from a plasmid made by: From pAN52 Dr P. Punt Netherlands.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Use of histone promoter to drive expression of secreted Xylanase C in Penicillium funiculosum. Also reference 12466887.