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pS6-TGFB2-STREP
(Plasmid #128503)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128503 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-Sport 6
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4396
  • Total vector size (bp) 5575
  • Modifications to backbone
    Introduction between SmaI site and L21 (tgfb2) of Kozak sequence, Igk leader and 2x-Strep tag.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TGFB2
  • Alt name
    Transforming growth factor beta-2 proprotein
  • Alt name
    Cetermin
  • Alt name
    Glioblastoma-derived T-cell suppressor factor
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1182
  • GenBank ID
    NP_001129071.1
  • Entrez Gene
    TGFB2 (a.k.a. G-TSF, LDS4, TGF-beta2)
  • Promoter CMV
  • Tag / Fusion Protein
    • STREP (2X) tag (N terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer GGTGGAGGTTCTGGAGGTGGAAGTGGAGGTAGCGCATGGAGCCATCCACAATTCGAAAAGCTCAGCCTGTCTACCTGC
  • 3′ sequencing primer CTTTTCGAATTGTGGATGGCTCCAGTCACCAGTGGAACCTGGAACCCAGAGCAG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pS6-TGFB2-STREP was a gift from F Xavier Gomis-Ruth (Addgene plasmid # 128503 ; http://n2t.net/addgene:128503 ; RRID:Addgene_128503)

  • For your References section:

    Recombinant production of human alpha2-macroglobulin variants and interaction studies with recombinant G-related alpha2-macroglobulin binding protein and latent transforming growth factor-beta2. Marino-Puertas L, Del Amo-Maestro L, Taules M, Gomis-Ruth FX, Goulas T. Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.1038/s41598-019-45712-z. 10.1038/s41598-019-45712-z PubMed 31235767
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