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pCri-H6-TEV-GRAB-STREP
(Plasmid #128499)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128499 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCri8a
  • Backbone manufacturer
    Homemade
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 5894
  • Modifications to backbone
    Introduction of STREP tag (2x)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GRAB (V34-N181)
  • Alt name
    Protein G-related alpha 2M-binding protein
  • Alt name
    SPy_1357
  • Species
    Streptococcus pyogenes serotype M1
  • Insert Size (bp)
    444
  • GenBank ID
    NP_269464.1
  • Entrez Gene
    grab (a.k.a. SPy_1357, SPy1357)
  • Promoter T7
  • Tags / Fusion Proteins
    • His tag (6x) (N terminal on insert)
    • Strep tag (2x) (C terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer ATGCCCATGGTTGATAGCCCG
  • 3′ sequencing primer See depositor comment
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Reverse primer 1: CGAATTGTGGATGGCTCCAACCTCCATTAACGTTCTGACGTTC

Reverse primer 2: CTTCCACCTCCAGAACCTCCACCCTTTTCGAATTGTGGATGGCTCC3

Reverse primer 3: GTGGATGGCTCCATGCGCTACCTCCACTTCCACCTCCAGAAC

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCri-H6-TEV-GRAB-STREP was a gift from F Xavier Gomis-Ruth (Addgene plasmid # 128499 ; http://n2t.net/addgene:128499 ; RRID:Addgene_128499)

  • For your References section:

    Recombinant production of human alpha2-macroglobulin variants and interaction studies with recombinant G-related alpha2-macroglobulin binding protein and latent transforming growth factor-beta2. Marino-Puertas L, Del Amo-Maestro L, Taules M, Gomis-Ruth FX, Goulas T. Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.1038/s41598-019-45712-z. 10.1038/s41598-019-45712-z PubMed 31235767
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