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pIE-hα2M-H6
(Plasmid #128492)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128492 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIEx
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3843
  • Total vector size (bp) 8190
  • Modifications to backbone
    Introduction of T after the signal peptide to introduce StuI restriction site = ATGTACAAGCTCACAGTCTTCCTGATGTTCATCGCTTTCGTCATCATCGCTGAGGCCt After AgeI restriction site; introductionn of 6x-His and stop codon = ACCGGTcatcatcaccatcaccatTGA
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    A2M
  • Alt name
    Alpha-2-macroglobulin
  • Alt name
    CPAMD5
  • Alt name
    FWP007
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4350
  • GenBank ID
    CR749334
  • Entrez Gene
    A2M (a.k.a. A2MD, CPAMD5, FWP007, S863-7)
  • Promoter IE1
  • Tag / Fusion Protein
    • His tag (6x) (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site StuI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer GGCCTCAGTCTCTGGAAAACCGCAGTATATG
  • 3′ sequencing primer ATTACCGGTAGGATTTCCAAGATCTTTG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIE-hα2M-H6 was a gift from F Xavier Gomis-Ruth (Addgene plasmid # 128492 ; http://n2t.net/addgene:128492 ; RRID:Addgene_128492)

  • For your References section:

    Recombinant production of human alpha2-macroglobulin variants and interaction studies with recombinant G-related alpha2-macroglobulin binding protein and latent transforming growth factor-beta2. Marino-Puertas L, Del Amo-Maestro L, Taules M, Gomis-Ruth FX, Goulas T. Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.1038/s41598-019-45712-z. 10.1038/s41598-019-45712-z PubMed 31235767
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