pIE-hα2M-H6
(Plasmid #128492)

• Purpose: Expresses human α2M (from S24 to N1473) in insect cells. With C-t H6x tag.
• Depositing Lab: F Xavier Gomis-Ruth
• Publication: Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.1038/s41598-019-45712-z.

Backbone

• Vector backbone: pIEx
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 3843
• Total vector size (bp): 8190
• Modifications to backbone: Introduction of T after the signal peptide to introduce StuI restriction site = ATGTACAAGCTCACAGTCTTCCTGATGTTCATCGCTTTCGTCATCATCGCTGAGGCCt After AgeI restriction site; introductionn of 6x-His and stop codon = ACCGGTcatcatcaccatcaccatTGA
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: A2M
• Alt name: Alpha-2-macroglobulin
• Alt name: CPAMD5
• Alt name: FWP007
• Species: H. sapiens (human)
• Insert Size (bp): 4350
• GenBank ID: CR749334
• Entrez Gene: A2M (a.k.a. A2MD, CPAMD5, FWP007, S863-7)
• Promoter: IE1
• Tag / Fusion Protein:
  • His tag (6x) (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: StuI (not destroyed)
• 3′ cloning site: AgeI (not destroyed)
• 5′ sequencing primer: GGCCTCAGTCTCTGGAAAACCGCAGTATATG
• 3′ sequencing primer: ATTACCGGTAGGATTTCCAAGATCTTTG

Resource Information

• Supplemental Documents:
  • Alpha2M_pIEx (S24-N1473).gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry