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Addgene

MSGV Mouse Acceptor PGK NGFR
(Plasmid #128482)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128482 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSGV1
  • Backbone manufacturer
    in lab construct
  • Backbone size (bp) 8052
  • Modifications to backbone
    TCR cloning insert: TCR V region gene acceptor/constant region genes tNGFR driven by PGK promoter
  • Vector type
    Retroviral
  • Promoter MSCV
  • Selectable markers
    truncated NGFR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    cDNA clone from I.M.A.G.E: 5263715

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSGV Mouse Acceptor PGK NGFR was a gift from David Ott (Addgene plasmid # 128482 ; http://n2t.net/addgene:128482 ; RRID:Addgene_128482)

  • For your References section:

    Production of retroviral constructs for effective transfer and expression of T-cell receptor genes using Golden Gate cloning. Coren LV, Jain S, Trivett MT, Ohlen C, Ott DE. Biotechniques. 2015 Mar 1;58(3):135-9. doi: 10.2144/000114265. eCollection 2015. 10.2144/000114265 PubMed 25757546
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