pSMPUW_6.16_ M4_Ef1a-gateway-V5-SV40-Zeo
(Plasmid
#128452)
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Purposelentiviral destination vector with Ef1a promoter, C-terminal V5 tag, SV40 promoter, and Zeo Resistance gene
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 128452 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSMPUW-eMCS
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Backbone manufacturerCell Biolabs, Inc
- Backbone size w/o insert (bp) 4796
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Kanamycin, 25 & 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)ccdB Survival
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameEf1a promoter, C-terminal V5 tag, SV40 promoter, and Zeocin Resistance gene
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSMPUW_6.16_ M4_Ef1a-gateway-V5-SV40-Zeo was a gift from Lindy Barrett (Addgene plasmid # 128452 ; http://n2t.net/addgene:128452 ; RRID:Addgene_128452)
