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Addgene

pSMPUW_6.14_M4_Ef1a-HA-gateway-SV40-Zeo
(Plasmid #128450)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128450 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pSMPUW-eMCS
  • Backbone manufacturer
    Cell Biolabs, Inc
  • Backbone size w/o insert (bp) 4796
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Kanamycin, 25 & 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Ef1a promoter, N-terminal HA tag, SV40 promoter, and Zeocin Resistance gene
  • Species
    Synthetic

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSMPUW_6.14_M4_Ef1a-HA-gateway-SV40-Zeo was a gift from Lindy Barrett (Addgene plasmid # 128450 ; http://n2t.net/addgene:128450 ; RRID:Addgene_128450)