pSMPUW_6.14_M4_Ef1a-HA-gateway-SV40-Zeo
(Plasmid
#128450)
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Purposelentiviral destination vector with Ef1a promoter, N-terminal HA tag, SV40 promoter, and Zeo Resistance gene
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 128450 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSMPUW-eMCS
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Backbone manufacturerCell Biolabs, Inc
- Backbone size w/o insert (bp) 4796
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Kanamycin, 25 & 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)ccdB Survival
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameEf1a promoter, N-terminal HA tag, SV40 promoter, and Zeocin Resistance gene
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSMPUW_6.14_M4_Ef1a-HA-gateway-SV40-Zeo was a gift from Lindy Barrett (Addgene plasmid # 128450 ; http://n2t.net/addgene:128450 ; RRID:Addgene_128450)