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pDONR_P4-P1R:PER36modp
(Plasmid #128431)

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Item Catalog # Description Quantity Price (USD)
Plasmid 128431 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pDONR_P4-P1R
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5892
  • Total vector size (bp) 5278
  • Vector type
    Gateway promoter entry clone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    For selection use 50 ug/ml Kanamycin
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PEROXISADE36
  • Alt name
    PER36
  • Alt name
    PRX36
  • Alt name
    At3g50990
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    2635
  • Mutation
    Premature stop codons introduced into the Xero1 (cytosine to thymine mutation at 1,560 bp) and Xero2 (guanine to thymine mutation at 345 bp) genes in the PER36 promoter insert
  • GenBank ID
    BT010535
  • Entrez Gene
    AT3G50990 (a.k.a. AT3G50990, PER36, peroxidase 36)
  • Promoter PEROXISADE36

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer 5’-TGTAAAACGACGGCCAGT-3
  • 3′ sequencing primer 5’-GAAGCATACCGAGCTAGAGC-3’
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The PER36 promoter (2,635 bp) was originally cloned into the pDONR P4-P1R entry clone as described in Kunieda T, Shimada T, Kondo M, Nishimura M, Nishitani K, Hara-Nishimura I (2013) Spatiotemporal Secretion of PEROXIDASE36 Is Required for Seed Coat Mucilage Extrusion in Arabidopsis. Plant Cell 25:1355–1367. doi: 10.1105/tpc.113.110072

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For use with Three-way Multi-site Gateway Cloning (Invitrogen).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR_P4-P1R:PER36modp was a gift from George Haughn (Addgene plasmid # 128431 ; http://n2t.net/addgene:128431 ; RRID:Addgene_128431)

  • For your References section:

    Assessing the utility of seed coat-specific promoters to engineer cell wall polysaccharide composition of mucilage. McGee R, Dean GH, Mansfield SD, Haughn GW. Plant Mol Biol. 2019 Aug 17. pii: 10.1007/s11103-019-00909-8. doi: 10.1007/s11103-019-00909-8. 10.1007/s11103-019-00909-8 PubMed 31422517
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