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pDONRG_P4-P1R:TBA2p
(Plasmid #128429)

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Item Catalog # Description Quantity Price (USD)
Plasmid 128429 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pDONRG_P4-P1R
  • Backbone manufacturer
    Invitrogen modified by doi:10.1271/bbb.70678
  • Backbone size w/o insert (bp) 5892
  • Total vector size (bp) 5104
  • Modifications to backbone
    151 bp within the attR1 sequence there is a thymine to cysteine modification which does not impact the gateway reaction.
  • Vector type
    Gateway promoter entry clone

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    For selection use 7 ug/ml Gentamicin
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TESTA ABUNDANT 2
  • Alt name
    TBA2
  • Alt name
    AT1G62060.1
  • Alt name
    O04575 (Uniprot)
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    1346
  • GenBank ID
    AY125570.1 NCBI GeneID: 842502
  • Entrez Gene
    AT1G62060 (a.k.a. AT1G62060, F19K23.1, F19K23_1)
  • Promoter TESTA ABUNDANT 2

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer M13F: 5’-TGTAAAACGACGGCCAGT-3’
  • 3′ sequencing primer M13R-pUC: 5’-CAGGAAACAGCTATGAC-3’
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For use with Three-way Multi-site Gateway Cloning (Invitrogen).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONRG_P4-P1R:TBA2p was a gift from George Haughn (Addgene plasmid # 128429 ; http://n2t.net/addgene:128429 ; RRID:Addgene_128429)

  • For your References section:

    Assessing the utility of seed coat-specific promoters to engineer cell wall polysaccharide composition of mucilage. McGee R, Dean GH, Mansfield SD, Haughn GW. Plant Mol Biol. 2019 Aug 17. pii: 10.1007/s11103-019-00909-8. doi: 10.1007/s11103-019-00909-8. 10.1007/s11103-019-00909-8 PubMed 31422517
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