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Addgene

pCS2+Lifeact-eGFP
(Plasmid #128428)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128428 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+CyclinB1-GFP
  • Backbone size w/o insert (bp) 4093
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Lifeact-eGFP
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    792
  • Promoter CMV & SP6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site BsrG1 (unknown if destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2+Lifeact-eGFP was a gift from Rusty Lansford (Addgene plasmid # 128428 ; http://n2t.net/addgene:128428 ; RRID:Addgene_128428)

  • For your References section:

    Fast and Efficient Expression of Multiple Proteins in Avian Embryos Using mRNA Electroporation. Tran M, Dave M, Lansford R. J Vis Exp. 2019 Jun 7;(148). doi: 10.3791/59664. 10.3791/59664 PubMed 31233018
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