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pcDNA3.1-dCas9-Dnmt3a-Dnmt3l-P2A-eGFP
(Plasmid #128424)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128424 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 12000
  • Modifications to backbone
    pcDNA3.1-dCas9-Dnmt3a-Dnmt3l-P2A-eGFP Human codon optimized dCas9-Dnmt3a-Dnmt3l
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dCas9m4-sv40NLS-DNMT3A-3L-sv40NLS-P2A-eGFP
  • Species
    Synthetic; Streptococcus pyogenes Type II
  • Insert Size (bp)
    6639
  • Promoter T7 Promoter
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer GAGCGGATAACAATTTCACACAGG
  • 3′ sequencing primer CGCAAATGGGCGGTAGGCGTG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-dCas9-Dnmt3a-Dnmt3l-P2A-eGFP was a gift from Bradley Bernstein (Addgene plasmid # 128424 ; http://n2t.net/addgene:128424 ; RRID:Addgene_128424)

  • For your References section:

    Epigenome editing strategies for the functional annotation of CTCF insulators. Tarjan DR, Flavahan WA, Bernstein BE. Nat Commun. 2019 Sep 18;10(1):4258. doi: 10.1038/s41467-019-12166-w. 10.1038/s41467-019-12166-w PubMed 31534142
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