pGEX-6P-1-INTS3-FL
(Plasmid
#128415)
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PurposeExpress INTS3 full-length in E. coli with a GST tag (N-terminal)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 128415 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEX-6P-1
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Backbone manufacturerGE Healthcare
- Backbone size w/o insert (bp) 4984
- Total vector size (bp) 8096
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameINTS3
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Alt nameSOSS-A
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Alt nameINT3
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Alt nameC1orf60
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SpeciesH. sapiens (human)
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Insert Size (bp)3129
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Mutationfull-length
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GenBank IDNM_023015.5
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Entrez GeneINTS3 (a.k.a. C1orf193, C1orf60, INT3, SOSS-A, SOSSA)
- Promoter tac promoter
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamH I (not destroyed)
- 3′ cloning site Xho I (not destroyed)
- 5′ sequencing primer pGEX 5' sequencing primer
- 3′ sequencing primer pGEX 3' sequencing primer (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX-6P-1-INTS3-FL was a gift from Yuliang Wu (Addgene plasmid # 128415 ; http://n2t.net/addgene:128415 ; RRID:Addgene_128415) -
For your References section:
Biochemical characterization of INTS3 and C9ORF80, two subunits of hNABP1/2 heterotrimeric complex in nucleic acid binding. Vidhyasagar V, He Y, Guo M, Talwar T, Singh RS, Yadav M, Katselis G, Vizeacoumar FJ, Lukong KE, Wu Y. Biochem J. 2018 Jan 2;475(1):45-60. doi: 10.1042/BCJ20170351. 10.1042/BCJ20170351 PubMed 29150435