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Addgene

pCZ47
(Plasmid #128414)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128414 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSL1190
  • Backbone size w/o insert (bp) 3400
  • Vector type
    Unspecified

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    vab-1
  • Alt name
    Ephrin Receptor
  • Species
    C. elegans (nematode)
  • Entrez Gene
    vab-1 (a.k.a. CELE_M03A1.1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer M13For
  • 3′ sequencing primer M13Rev
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Made by Sean George in the Chisholm Lab
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCZ47 was a gift from Andrew Chisholm (Addgene plasmid # 128414 ; http://n2t.net/addgene:128414 ; RRID:Addgene_128414)

  • For your References section:

    The VAB-1 Eph receptor tyrosine kinase functions in neural and epithelial morphogenesis in C. elegans. George SE, Simokat K, Hardin J, Chisholm AD. Cell. 1998 Mar 6;92(5):633-43. PubMed 9506518
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