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Addgene

pNM58
(Plasmid #128409)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128409 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pXyl-AgrCA1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Need to grow cell in minimal medium (S750) with 1% arabinose. This plasmid doesn't work in LB.
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    argCA
  • Species
    Staphylococcus aureus
  • Promoter PxylR

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer gacttgtttaatcctgtaatctcagagagag
  • 3′ sequencing primer acttcgcagattattctatactgtgctaac
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    gfpmut2
  • Species
    Synthetic; Staphylococcus aureus
  • Promoter P3

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer cctttaccttgtctacaaacccc
  • 3′ sequencing primer GCTGAAGTCAAGTTTGAAGGTGATACC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNM58 was a gift from Christopher Voigt (Addgene plasmid # 128409 ; http://n2t.net/addgene:128409 ; RRID:Addgene_128409)

  • For your References section:

    Resilient living materials built by printing bacterial spores. Gonzalez LM, Mukhitov N, Voigt CA. Nat Chem Biol. 2019 Dec 2. pii: 10.1038/s41589-019-0412-5. doi: 10.1038/s41589-019-0412-5. 10.1038/s41589-019-0412-5 PubMed 31792444
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