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pCI118-SiT-Cas12a-[Ind]
(Plasmid #128406)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128406 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-MCS
  • Backbone size w/o insert (bp) 4800
  • Total vector size (bp) 9300
  • Modifications to backbone
    Tet inducible promoter
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    AsCas12a-Triplex
  • Alt name
    Cpf1
  • Insert Size (bp)
    4300

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Feng Zhang Lab (py108)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI118-SiT-Cas12a-[Ind] was a gift from Randall Platt (Addgene plasmid # 128406 ; http://n2t.net/addgene:128406 ; RRID:Addgene_128406)

  • For your References section:

    Multiplexed genome engineering by Cas12a and CRISPR arrays encoded on single transcripts. Campa CC, Weisbach NR, Santinha AJ, Incarnato D, Platt RJ. Nat Methods. 2019 Aug 12. pii: 10.1038/s41592-019-0508-6. doi: 10.1038/s41592-019-0508-6. 10.1038/s41592-019-0508-6 PubMed 31406383
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