pSF-CjPglB-LpxE
(Plasmid #128389)

• Purpose: Expression of C. jejuni PglB and F. tularensis LpxE in E. coli
• Depositing Lab: Michael Jewett
• Publication: Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9444. doi: 10.1126/sciadv.abe9444. Print 2021 Feb.

Backbone

• Vector backbone: pSF
• Backbone size w/o insert (bp): 3720
• Total vector size (bp): 6976
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: PglB oligosaccharylktransferase
• Species: C. jejuni
• Insert Size (bp): 2139
• Promoter: pBAD
• Tag / Fusion Protein:
  • Flag tag (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: CGCTTTTTATCGCAACTCTCTACTG
• 3′ sequencing primer: ACAGCCAAGCTGGAGACCG

Gene/Insert 2

• Gene/Insert name: LpxE phosphatase
• Species: F. tularensis
• Insert Size (bp): 720
• Promoter: Ptac

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: NsiI (not destroyed)
• 5′ sequencing primer: GGAATTGTGAGCGGATAACAATTTCAC
• 3′ sequencing primer: GCAGTTAGGGATTAGCGTCTTAAGC

Resource Information

• Supplemental Documents:
  • pSF-CjPglB-LpxE.gb
• A portion of this plasmid was derived from a plasmid made by: pSF CjPglB backbone is from Ollis, et al. doi.org/10.1038/srep15237; F. tularensis LpxE gene is from pE Needham, et al. doi.org/10.1073/pnas.1218080110

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry