pLenti NLuc398-hLGALS8 IRES hCALCOCO2-CLuc394
(Plasmid #128387)

• Purpose: Expresses a split firefly luciferase reporter based on the full length, human Gal8 and CALCOCO2, which interact following endosomal disruption
• Depositing Lab: Craig Duvall
• Publication: Kilchrist et al ACS Sens. 2020 Jun 23. doi: 10.1021/acssensors.0c00103.

Backbone

• Vector backbone: pLV
• Backbone manufacturer: VectorBuilder
• Backbone size w/o insert (bp): 7344
• Total vector size (bp): 12269
• Vector type: Mammalian Expression, Lentiviral, Luciferase, Synthetic Biology
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: NLuc398-3xGGGGS-LGALS8
• Alt name: Gal8
• Alt name: Galectin 8
• Species: H. sapiens (human), Synthetic
• GenBank ID: NM_201545.2
• Entrez Gene: LGALS8 (a.k.a. Gal-8, PCTA-1, PCTA1, Po66-CBP)
• Promoter: EFS
• Tag / Fusion Protein:
  • NLuc398 (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: None

Gene/Insert 2

• Gene/Insert name: CALCOCO2-3xGGGGS-CLuc394
• Alt name: CALCOCO2
• Alt name: NDP52
• Species: H. sapiens (human)
• GenBank ID: NM_001261390.1
• Entrez Gene: CALCOCO2 (a.k.a. NDP52)
• Promoter: IRES
• Tag / Fusion Protein:
  • CLuc394 (C terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: None

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Outsourced synthesis

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry