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pGEX-UbcH7(C86K)-10aa-linker-delta 1-29 and 117-160 Bactrocera parkin(C463A)
(Plasmid #128383)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128383 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-6P1
  • Backbone manufacturer
    GE Healthcare
  • Backbone size w/o insert (bp) 4960
  • Total vector size (bp) 6727
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    lower temperature to 16C for protein expression in BL21
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    UbcH7
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1767
  • Mutation
    mutation C86K in UbcH7 gene
  • Entrez Gene
    UBE2L3 (a.k.a. E2-F1, L-UBC, UBCH7, UbcM4)
  • Promoter lac promotor
  • Tags / Fusion Proteins
    • GST (N terminal on backbone)
    • Bactrocera dorsalis parkin with mutation C463A and deleted residues 1 to 29 and 117 to 160 (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGgatccATGGCGGCCAGCAGGAGGCTG
  • 3′ sequencing primer gccaccgccggatccctggaagtacaggttttcaccgccGTCCACAGGTCGCTTTTCCCC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-UbcH7(C86K)-10aa-linker-delta 1-29 and 117-160 Bactrocera parkin(C463A) was a gift from Kalle Gehring (Addgene plasmid # 128383 ; http://n2t.net/addgene:128383 ; RRID:Addgene_128383)

  • For your References section:

    Mechanism of parkin activation by phosphorylation. Sauve V, Sung G, Soya N, Kozlov G, Blaimschein N, Miotto LS, Trempe JF, Lukacs GL, Gehring K. Nat Struct Mol Biol. 2018 Jul;25(7):623-630. doi: 10.1038/s41594-018-0088-7. Epub 2018 Jul 2. 10.1038/s41594-018-0088-7 PubMed 29967542
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