pHDR mPGK-puroTK
(Plasmid
#128382)
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PurposeHomology dependent repair construct with positive selection puromycin and negative selection viral thymidine kinase cassettes
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 128382 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC57
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Vector typehomology-directed repair plasmid
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHDR, puromycin and viral thymidine kinase
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Alt nameHDR +/- selection cassette
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHDR mPGK-puroTK was a gift from Budd A. Tucker (Addgene plasmid # 128382) -
For your References section:
CRISPR-Cas9-Based Genome Editing of Human Induced Pluripotent Stem Cells. Giacalone JC, Sharma TP, Burnight ER, Fingert JF, Mullins RF, Stone EM, Tucker BA. Curr Protoc Stem Cell Biol. 2018 Feb 28;44:5B.7.1-5B.7.22. doi: 10.1002/cpsc.46. 10.1002/cpsc.46 PubMed 29512106