pPEE13
(Plasmid #128359)

• Purpose: Recycable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mCherry ORF and a flexable linker at the 5' end.
• Depositing Lab: James Fraser
• Publication: Erpf et al Fungal Genet Biol. 2019 Jun 17;131:103241. doi: 10.1016/j.fgb.2019.103241.

Backbone

• Vector backbone: pBlueScript II SK(-)
• Backbone manufacturer: Stratagene
• Vector type: Unspecified
• Selectable markers: Acetamide, Fluoroacetamide

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Acetamidase (amdS)
• Species: Synthetic
• Promoter: TEF1

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • pPEE13.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Contains the amdS2 mCherry Blaster marker with a flexable linker in the multicloning site of pBluescript II SK-. Importantly, the amdS2 marker can be excised using restriction enzymes HindIII and EcoRI.