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Addgene

pPEE8
(Plasmid #128358)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128358 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBlueScript II SK(-)
  • Backbone manufacturer
    Stratagene
  • Vector type
    Unspecified
  • Selectable markers
    Acetamide, Fluoroacetamide

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Acetamidase (amdS)
  • Species
    Synthetic
  • Promoter TEF1

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Contains the amdS2 Blaster marker in the multicloning site of pBluescript II SK-. Importantly, the amdS marker can be excised using restriction enzymes HindIII and SpeI.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPEE8 was a gift from James Fraser (Addgene plasmid # 128358 ; http://n2t.net/addgene:128358 ; RRID:Addgene_128358)

  • For your References section:

    amdS as a dominant recyclable marker in Cryptococcus neoformans. Erpf PE, Stephenson CJ, Fraser JA. Fungal Genet Biol. 2019 Jun 17;131:103241. doi: 10.1016/j.fgb.2019.103241. 10.1016/j.fgb.2019.103241 PubMed 31220607
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