pPEE7
(Plasmid
#128357)
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Purpose(Empty Backbone) Recycable marker for use in Cryptococcus neoformans. Contains the amdS1 counterselectable marker flanked by direct repeats.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 128357 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBlueScript II SK(-)
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Backbone manufacturerStratagene
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Vector typeUnspecified
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Selectable markersAcetamide, Fluoroacetamide
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAcetamidase (amdS)
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SpeciesSynthetic
- Promoter ACT1
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Contains the amdS1 Blaster marker in the multicloning site of pBluescript II SK-. Importantly, the amdS marker can be excised using restriction enzymes HindIII and SpeI.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPEE7 was a gift from James Fraser (Addgene plasmid # 128357 ; http://n2t.net/addgene:128357 ; RRID:Addgene_128357) -
For your References section:
amdS as a dominant recyclable marker in Cryptococcus neoformans. Erpf PE, Stephenson CJ, Fraser JA. Fungal Genet Biol. 2019 Jun 17;131:103241. doi: 10.1016/j.fgb.2019.103241. 10.1016/j.fgb.2019.103241 PubMed 31220607
Map uploaded by the depositor.
