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Addgene

pSDMA65
(Plasmid #128354)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128354 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBlueScript II SK(-)
  • Backbone manufacturer
    Stratagene
  • Vector type
    CRISPR
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Hygromycin (HYG)
  • Species
    Synthetic
  • Promoter ACT1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSDMA65 was a gift from James Fraser (Addgene plasmid # 128354 ; http://n2t.net/addgene:128354 ; RRID:Addgene_128354)

  • For your References section:

    Targeted Genome Editing via CRISPR in the Pathogen Cryptococcus neoformans. Arras SD, Chua SM, Wizrah MS, Faint JA, Yap AS, Fraser JA. PLoS One. 2016 Oct 6;11(10):e0164322. doi: 10.1371/journal.pone.0164322. eCollection 2016. 10.1371/journal.pone.0164322 PubMed 27711143
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