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Addgene

pQCXIP-mCherry-TAZ
(Plasmid #128351)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128351 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQCXIP
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TAZ
  • Alt name
    WWTR1
  • Species
    H. sapiens (human)
  • Entrez Gene
    WWTR1 (a.k.a. TAZ)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer Ggcatggacgagctgtacaa
  • 3′ sequencing primer ccctaggaatgctcgtcaag
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pCIneomCherry-TAZ was generated by ligating TAZ(EcoRI/XhoI) into EcoRI/Sall sites of pCIneomCherry. NheI/NotI fragment was ligated inot XbaI/NotI sites of pQCXIP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQCXIP-mCherry-TAZ was a gift from Yutaka Hata (Addgene plasmid # 128351 ; http://n2t.net/addgene:128351 ; RRID:Addgene_128351)
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