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pCIneoLuc-PP2A
(Plasmid #128349)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128349 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCIneo
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PP2A
  • Alt name
    PTPA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1080
  • GenBank ID
    5524
  • Entrez Gene
    PTPA (a.k.a. PARK25, PP2A, PPP2R4, PR53)
  • Promoter CMV
  • Tag / Fusion Protein
    • Luciferase (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer gataggcacctattggtcttactgacat
  • 3′ sequencing primer Gaacctgaaacataaaatgaat
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The PP2A insert contains a H804Q mismatch compared to the NCBI reference.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCIneoLuc-PP2A was a gift from Yutaka Hata (Addgene plasmid # 128349 ; http://n2t.net/addgene:128349 ; RRID:Addgene_128349)

  • For your References section:

    A cell-based screening for TAZ activators identifies ethacridine, a widely used antiseptic and abortifacient, as a compound that promotes dephosphorylation of TAZ and inhibits adipogenesis in C3H10T1/2 cells. Kawano S, Maruyama J, Nagashima S, Inami K, Qiu W, Iwasa H, Nakagawa K, Ishigami-Yuasa M, Kagechika H, Nishina H, Hata Y. J Biochem. 2015 May 14. pii: mvv051. 10.1093/jb/mvv051 PubMed 25979969
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