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pFB-U6-Chrnb4-gRNA-hSyn-mCherry-WPRE-SV40pA
(Plasmid #128345)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128345 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MLM3636
  • Backbone manufacturer
    Keith Joung (Addgene plasmid #43860)
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Chrnb4
  • gRNA/shRNA sequence
    GGACTGACTACCGCCTGGCC
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Chrnb4 (a.k.a. Acrb-4, Acrb4)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFB-U6-Chrnb4-gRNA-hSyn-mCherry-WPRE-SV40pA was a gift from Ryan Drenan (Addgene plasmid # 128345 ; http://n2t.net/addgene:128345 ; RRID:Addgene_128345)

  • For your References section:

    Gene editing vectors for studying nicotinic acetylcholine receptors in cholinergic transmission. Peng C, Yan Y, Kim VJ, Engle SE, Berry JN, McIntosh JM, Neve RL, Drenan RM. Eur J Neurosci. 2018 May 19. doi: 10.1111/ejn.13957. 10.1111/ejn.13957 PubMed 29779223
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