dCas9 gateway compatible
(Plasmid
#128322)
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Purpose(Empty Backbone) dCas9 C-terminal gateway cloning vector
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 128322 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.3-TOPO
- Backbone size (bp) 11000
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Modifications to backboneInserted gateway cloning site into https://www.addgene.org/47316/
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Vector typeMammalian Expression, Synthetic Biology
- Promoter CMV
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
dCas9 gateway compatible was a gift from Alejandro Chavez (Addgene plasmid # 128322 ; http://n2t.net/addgene:128322 ; RRID:Addgene_128322)
