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pET29a-hNABP2NhNABP1C
(Plasmid #128313)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128313 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET29a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5371
  • Total vector size (bp) 5829
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    chimeric protein hNABP2N+hNABP1C
  • Alt name
    NABP1
  • Alt name
    NABP2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    588
  • Mutation
    chimeric protein hNABP2N+hNABP1C, Y85C (please see depositor comments)
  • GenBank ID
    NM_024068.4 NM_001031716.3
  • Entrez Gene
    NABP2 (a.k.a. OBFC2B, SOSS-B1, SSB1, hSSB1)
  • Entrez Gene
    NABP1 (a.k.a. OBFC2A, SOSS-B2, SSB2)
  • Promoter T7
  • Tag / Fusion Protein
    • His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nde I (not destroyed)
  • 3′ cloning site Xho I (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Y85C mutation does not affect plasmid function. Point mutation is located in the middle of the OB fold, and no particular function is reported/known for this residue.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET29a-hNABP2NhNABP1C was a gift from Yuliang Wu (Addgene plasmid # 128313 ; http://n2t.net/addgene:128313 ; RRID:Addgene_128313)

  • For your References section:

    C-termini are essential and distinct for nucleic acid binding of human NABP1 and NABP2. Vidhyasagar V, He Y, Guo M, Ding H, Talwar T, Nguyen V, Nwosu J, Katselis G, Wu Y. Biochim Biophys Acta. 2016 Feb;1860(2):371-83. doi: 10.1016/j.bbagen.2015.11.003. Epub 2015 Nov 10. 10.1016/j.bbagen.2015.11.003 PubMed 26550690
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