pET29a-hNABP1NhNABP2C
(Plasmid
#128312)
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PurposeExpress chimeric protein hNABP1 N-terminus and hNABP2 C-terminus in E. coli with a C-terminal His-tag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 128312 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET29a
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5371
- Total vector size (bp) 5880
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namechimeric protein hNABP1N+hNABP2C
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Alt nameNABP1
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Alt nameNABP2
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SpeciesH. sapiens (human)
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Insert Size (bp)639
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Mutationchimeric protein hNABP1N+hNABP2C
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GenBank IDNM_001031716.3 NM_024068.4
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Entrez GeneNABP1 (a.k.a. OBFC2A, SOSS-B2, SSB2)
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Entrez GeneNABP2 (a.k.a. OBFC2B, SOSS-B1, SSB1, hSSB1)
- Promoter T7
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Tag
/ Fusion Protein
- His (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Nde I (not destroyed)
- 3′ cloning site Xho I (not destroyed)
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET29a-hNABP1NhNABP2C was a gift from Yuliang Wu (Addgene plasmid # 128312 ; http://n2t.net/addgene:128312 ; RRID:Addgene_128312) -
For your References section:
C-termini are essential and distinct for nucleic acid binding of human NABP1 and NABP2. Vidhyasagar V, He Y, Guo M, Ding H, Talwar T, Nguyen V, Nwosu J, Katselis G, Wu Y. Biochim Biophys Acta. 2016 Feb;1860(2):371-83. doi: 10.1016/j.bbagen.2015.11.003. Epub 2015 Nov 10. 10.1016/j.bbagen.2015.11.003 PubMed 26550690