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pET29a-hNABP1-N
(Plasmid #128310)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128310 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET29a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5371
  • Total vector size (bp) 5658
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hNABP1 N-terminal domain (OB fold, 1–141 aa)
  • Alt name
    hSSB2
  • Species
    H. sapiens (human)
  • Mutation
    N-terminal domain (OB fold, 1–140 aa)
  • GenBank ID
    NM_001031716.3
  • Entrez Gene
    NABP1 (a.k.a. OBFC2A, SOSS-B2, SSB2)
  • Promoter T7
  • Tag / Fusion Protein
    • His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nde I (not destroyed)
  • 3′ cloning site Xho I (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET29a-hNABP1-N was a gift from Yuliang Wu (Addgene plasmid # 128310 ; http://n2t.net/addgene:128310 ; RRID:Addgene_128310)

  • For your References section:

    C-termini are essential and distinct for nucleic acid binding of human NABP1 and NABP2. Vidhyasagar V, He Y, Guo M, Ding H, Talwar T, Nguyen V, Nwosu J, Katselis G, Wu Y. Biochim Biophys Acta. 2016 Feb;1860(2):371-83. doi: 10.1016/j.bbagen.2015.11.003. Epub 2015 Nov 10. 10.1016/j.bbagen.2015.11.003 PubMed 26550690
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