pET29a-hNABP2
(Plasmid #128307)

• Purpose: Express hNABP2 (a.k.a hSSB1 or SOSSB1) in E. coli with a C-terminal His-tag
• Depositing Lab: Yuliang Wu
• Publication: Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):371-83. doi: 10.1016/j.bbagen.2015.11.003. Epub 2015 Nov 10.

Backbone

• Vector backbone: pET29a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5371
• Total vector size (bp): 5871
• Modifications to backbone: No
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Human Nucleic Acid Binding Protein 2 (hNABP2)
• Alt name: human single-stranded DNA binding protein (hSSB1)
• Alt name: SOSSB1
• Species: H. sapiens (human)
• Insert Size (bp): 630
• Mutation: Y85C- please see depositor comments below
• GenBank ID: NM_024068.4
• Entrez Gene: NABP2 (a.k.a. OBFC2B, SOSS-B1, SSB1, hSSB1)
• Promoter: T7
• Tag / Fusion Protein:
  • His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nde I (not destroyed)
• 3′ cloning site: Xho I (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Y85C mutation does not affect plasmid function. Point mutation is located in the middle of the OB fold, and no particular function is reported/known for this residue.