p2HGPD/ER/cyc
(Plasmid
#1283)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 1283 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbone2u (HIS3)
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameER
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Alt nameestrogen receptor
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SpeciesS. cerevisiae (budding yeast)
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Tag
/ Fusion Protein
- Gpd promoter (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p2HGPD/ER/cyc was a gift from Susan Lindquist (Addgene plasmid # 1283 ; http://n2t.net/addgene:1283 ; RRID:Addgene_1283) -
For your References section:
Role of the protein chaperone YDJ1 in establishing Hsp90-mediated signal transduction pathways. Kimura Y, Yahara I, Lindquist S. Science 1995 Jun 2;268(5215):1362-5. 10.1126/science.7761857 PubMed 7761857