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Addgene

pEE29
(Plasmid #128287)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128287 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CloDF13
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Piq - sfyfp, sopC

Cloning Information

  • Cloning method Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cloning method: Golden Gate.

Please note: Plasmid contains a G163C mutation in LacZ. This mutation is not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEE29 was a gift from Matthew Bennett (Addgene plasmid # 128287 ; http://n2t.net/addgene:128287 ; RRID:Addgene_128287)

  • For your References section:

    A synthetic system for asymmetric cell division in Escherichia coli. Molinari S, Shis DL, Bhakta SP, Chappell J, Igoshin OA, Bennett MR. Nat Chem Biol. 2019 Sep;15(9):917-924. doi: 10.1038/s41589-019-0339-x. Epub 2019 Aug 12. 10.1038/s41589-019-0339-x PubMed 31406375
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