pCC48
(Plasmid
#128285)
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Purposeasymmetric plasmid partitioning E. coli - target plasmid
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 128285 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMB1+rop
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePiq - mRFP, BBa_J23100 - phlF, parS
Cloning Information
- Cloning method Unknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Cloning method: Golden Gate.
Please note: Plasmid contains a T180A mutation in mRFP. This mutation is not known to affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCC48 was a gift from Matthew Bennett (Addgene plasmid # 128285 ; http://n2t.net/addgene:128285 ; RRID:Addgene_128285) -
For your References section:
A synthetic system for asymmetric cell division in Escherichia coli. Molinari S, Shis DL, Bhakta SP, Chappell J, Igoshin OA, Bennett MR. Nat Chem Biol. 2019 Sep;15(9):917-924. doi: 10.1038/s41589-019-0339-x. Epub 2019 Aug 12. 10.1038/s41589-019-0339-x PubMed 31406375