pCMS-dsRed2-C1824U LMNA-GFP minigene
(Plasmid #128231)

• Purpose: C1824U mutation Lamin A splicing reporter
• Depositing Lab: Tom Misteli
• Publication: Shilo et al Nucleic Acids Res. 2019 Apr 22. pii: 5475939. doi: 10.1093/nar/gkz259.

Backbone

• Vector backbone: pCMS-EGFP
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 6350
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: lamin A
• Alt name: LMNA
• Species: H. sapiens (human)
• Insert Size (bp): 850
• Mutation: C1824U
• Entrez Gene: LMNA (a.k.a. CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1)
• Promoter: CMV
• Tags / Fusion Proteins:
  • GFP (C terminal on backbone)
  • SV40-driven dsRed2 to identify transfected cells.

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EGFP-N

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Paula Scaffidi

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

lamin A splicing reporter with short-lived GFP downstream LMNA exon 12 (CMV-driven) + SV40-driven dsRed2 to identified transfected cells. C>T mutation in exon 11. The mutation causes aberrant splicing event, creating a mutant protein, delta50 lamin A.