pFH54
(Plasmid
#128183)
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PurposeLevel1 AtUBI10p::SpCas9 (Arabidopsis codon optimized):pea3At
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 128183 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepICH47742
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Vector typePlant Expression, CRISPR, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFH54 was a gift from Vladimir Nekrasov (Addgene plasmid # 128183 ; http://n2t.net/addgene:128183 ; RRID:Addgene_128183) -
For your References section:
A modular cloning toolkit for genome editing in plants. Hahn F, Korolev A, Sanjurjo Loures L, Nekrasov V. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. 10.1186/s12870-020-02388-2 PubMed 32326901