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Addgene

NM9-SpCas9-NLS3
(Plasmid #128177)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128177 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAMBRIA
  • Backbone manufacturer
    Zhao lab
  • Backbone size w/o insert (bp) 13658
  • Total vector size (bp) 17762
  • Vector type
    Yeast Expression, CRISPR
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpCas9-NLS3
  • Species
    Rhodosporidium toruloides
  • Insert Size (bp)
    4182
  • Promoter pPGK1

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer CAAATTGACGCTTAGACAAC
  • 3′ sequencing primer TATATCCTGTCAAACACTGATAG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NM9-SpCas9-NLS3 was a gift from Huimin Zhao (Addgene plasmid # 128177 ; http://n2t.net/addgene:128177 ; RRID:Addgene_128177)

  • For your References section:

    Development of a CRISPR/Cas9 system for high efficiency multiplexed gene deletion in Rhodosporidium toruloides. Schultz JC, Cao M, Zhao H. Biotechnol Bioeng. 2019 Apr 30. doi: 10.1002/bit.27001. 10.1002/bit.27001 PubMed 31038202
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