MSGV Mouse Acceptor
(Plasmid
#128176)
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Purpose(Empty Backbone) A TCR Variable Region cloning expression vector using Mouse Constant Regions to reduce mis-pairing with endogenous TCR ref Biotechniques. 2015 Mar 1;58(3):135-9
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 128176 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneMSGV1
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Backbone manufacturerin lab construct
- Backbone size (bp) 6679
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Vector typeRetroviral
- Promoter MSCV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MSGV Mouse Acceptor was a gift from David Ott (Addgene plasmid # 128176 ; http://n2t.net/addgene:128176 ; RRID:Addgene_128176) -
For your References section:
Production of retroviral constructs for effective transfer and expression of T-cell receptor genes using Golden Gate cloning. Coren LV, Jain S, Trivett MT, Ohlen C, Ott DE. Biotechniques. 2015 Mar 1;58(3):135-9. doi: 10.2144/000114265. eCollection 2015. 10.2144/000114265 PubMed 25757546
Map uploaded by the depositor.
