pCAGGS-mTagBFP2-T2A-sfGFP-iCre-ERT2
(Plasmid #128166)

• Purpose: Monomeric TagBFP2 and sfGFP-iCre-ERT2 recombinase (PAPGSTM N-terminus, unskippable linker)
• Depositing Lab: Mario Capecchi
• Publication: Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10.1186/s13064-020-00139-5.

Backbone

• Vector backbone: pCAGGS
• Vector type: Mammalian Expression, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mTagBFP2-T2A-sfGFP-iCre-ERT2
• Species: Synthetic

Resource Information

• Supplemental Documents:
  • pCX-mTagBFP2-mT2A-sfGFP-iCreERT2.gb
• A portion of this plasmid was derived from a plasmid made by: pCAGGS is from BCCM/LMBP. mTagBFP2 is from Michael Davidson. sfGFP is from Sandia Biotech. iCre is from Rolf Sprengel. ERT2 is from Pierre Chambon.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Linker between sfGFP and iCre-ERT2 is a mutated P2A sequence where 2 conserved amino acids are changed to Alanines. This mutated sequence does not skip.

The sfGFP fluorescence from the sfGFP-iCre-ERT2 is dim, as described in Nam and Capecchi, 2020 (https://doi.org/10.1186/s13064-020-00139-5) and a Figshare page (https://doi.org/10.6084/m9.figshare.13218437.v35) In short, it is not visible to the eyes, but can be detected with flow cytometry.