pCAH01
(Plasmid
#128162)
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Purpose(Empty Backbone) IncP-based broad host range vector used for inducible expression of genes in methanotrophic bacteria
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 128162 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAWP78
- Backbone size (bp) 6400
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Modifications to backboneA ~2400bp fragment containing the inducible tetracycline promoter/operator and tetR from pASK75 was cloned via Gibson Assembly to generate a broad host range inducible expression vector
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Vector typeBacterial Expression
- Promoter Ptet
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Selectable markersampicillin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CCC GAC ACC ATC GAA TGG CCA GAT G
- 3′ sequencing primer CAG GGC GCG TGG AGA TCC GT (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pAWP78 was gifted from Mary Lidstrom's laboratory at the University of Washington
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAH01 was a gift from Michael Guarnieri & Calvin Henard (Addgene plasmid # 128162 ; http://n2t.net/addgene:128162 ; RRID:Addgene_128162) -
For your References section:
Bioconversion of methane to lactate by an obligate methanotrophic bacterium. Henard CA, Smith H, Dowe N, Kalyuzhnaya MG, Pienkos PT, Guarnieri MT. Sci Rep. 2016 Feb 23;6:21585. doi: 10.1038/srep21585. 10.1038/srep21585 PubMed 26902345
Map uploaded by the depositor.
