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Addgene

pCAH01Sp::Cas9D10A
(Plasmid #128161)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128161 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAH01SpR
  • Backbone size w/o insert (bp) 7755
  • Total vector size (bp) 11704
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Sp Cas9
  • Species
    S. pyogenes
  • Insert Size (bp)
    4107
  • Mutation
    D10A
  • Promoter Ptet

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CCC GAC ACC ATC GAA TGG CCA GAT G
  • 3′ sequencing primer CAG GGC GCG TGG AGA TCC GT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAH01Sp::Cas9D10A was a gift from Calvin Henard (Addgene plasmid # 128161 ; http://n2t.net/addgene:128161 ; RRID:Addgene_128161)

  • For your References section:

    Development of a CRISPR/Cas9 System for Methylococcus capsulatus In Vivo Gene Editing. Tapscott T, Guarnieri MT, Henard CA. Appl Environ Microbiol. 2019 May 16;85(11). pii: AEM.00340-19. doi: 10.1128/AEM.00340-19. Print 2019 Jun 1. 10.1128/AEM.00340-19 PubMed 30926729
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