pCAH01SpR
(Plasmid
#128159)
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Purpose(Empty Backbone) pCAH01 derivative (Ptet inducible promoter) with ahp (kanamycin resistance) CDS replaced with the aadA (streptomycin/spectinomycin resistance) CDS
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 128159 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCAH01
- Backbone size (bp) 7600
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Modifications to backboneThe ahp CDS was replaced with the aadA CDS via Gibson cloning.
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Vector typeBacterial Expression
- Promoter Ptet
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAH01SpR was a gift from Calvin Henard (Addgene plasmid # 128159 ; http://n2t.net/addgene:128159 ; RRID:Addgene_128159) -
For your References section:
Development of a CRISPR/Cas9 System for Methylococcus capsulatus In Vivo Gene Editing. Tapscott T, Guarnieri MT, Henard CA. Appl Environ Microbiol. 2019 May 16;85(11). pii: AEM.00340-19. doi: 10.1128/AEM.00340-19. Print 2019 Jun 1. 10.1128/AEM.00340-19 PubMed 30926729
Map uploaded by the depositor.
