SarI T39N YFP
(Plasmid
#128155)
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PurposeExpression of Sar1 with T39N dominant negative mutation
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 128155 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSar1
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MutationT39N
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Tag
/ Fusion Protein
- EYFP (C terminal on backbone)
Cloning Information
- Cloning method Unknown
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Sar1-T39N is a dominant-negative mutant
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SarI T39N YFP was a gift from Jennifer Lippincott-Schwartz (Addgene plasmid # 128155 ; http://n2t.net/addgene:128155 ; RRID:Addgene_128155) -
For your References section:
ER-to-Golgi protein delivery through an interwoven, tubular network extending from ER. Weigel AV, Chang CL, Shtengel G, Xu CS, Hoffman DP, Freeman M, Iyer N, Aaron J, Khuon S, Bogovic J, Qiu W, Hess HF, Lippincott-Schwartz J. Cell. 2021 Apr 29;184(9):2412-2429.e16. doi: 10.1016/j.cell.2021.03.035. Epub 2021 Apr 13. 10.1016/j.cell.2021.03.035 PubMed 33852913