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Addgene

SarI T39N YFP
(Plasmid #128155)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128155 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEGFP
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Sar1
  • Mutation
    T39N
  • Tag / Fusion Protein
    • EYFP (C terminal on backbone)

Cloning Information

  • Cloning method Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Sar1-T39N is a dominant-negative mutant

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SarI T39N YFP was a gift from Jennifer Lippincott-Schwartz (Addgene plasmid # 128155 ; http://n2t.net/addgene:128155 ; RRID:Addgene_128155)
  • For your References section:

    ER-to-Golgi protein delivery through an interwoven, tubular network extending from ER. Weigel AV, Chang CL, Shtengel G, Xu CS, Hoffman DP, Freeman M, Iyer N, Aaron J, Khuon S, Bogovic J, Qiu W, Hess HF, Lippincott-Schwartz J. Cell. 2021 Apr 29;184(9):2412-2429.e16. doi: 10.1016/j.cell.2021.03.035. Epub 2021 Apr 13. 10.1016/j.cell.2021.03.035 PubMed 33852913