RFP Dynamin2 K44A
(Plasmid #128153)

• Purpose: Expression of Dynamin 2 K44A
• Depositing Lab: Jennifer Lippincott-Schwartz
• Publication: Chua et al Proc Natl Acad Sci U S A. 2009 Nov 30. ():.

Backbone

• Vector backbone: pEGFP
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Dynamin 2
• Species: R. norvegicus (rat)
• Mutation: K44A
• Entrez Gene: Dnm2 (a.k.a. DYIIAAB)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: HindIII (unknown if destroyed)

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: Plasmid contains D406G, V664A, H692R, and Q758R mutations in Dynamin2 compared to the NCBI reference sequence XP_006242670.1. These mutations are not known to affect plasmid function.