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Addgene

DinoIII-arrO-N
(Plasmid #128143)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128143 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMD19 T-Vector
  • Backbone manufacturer
    Takara
  • Backbone size w/o insert (bp) 2679
  • Total vector size (bp) 5660
  • Vector type
    Bacterial Expression
  • Selectable markers
    Rifampin ADP-ribosylating transferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rifampin ADP-ribosylating transferase + potential promoter
  • Alt name
    arrO
  • Species
    Codon optimized for Oxyrrhis marina
  • Insert Size (bp)
    523

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (destroyed during cloning)
  • 3′ cloning site BglII (destroyed during cloning)
  • 5′ sequencing primer M13R
  • 3′ sequencing primer M13F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DinoIII-arrO-N was a gift from Senjie Lin (Addgene plasmid # 128143 ; http://n2t.net/addgene:128143 ; RRID:Addgene_128143)

  • For your References section:

    Nuclear Gene Transformation in the Dinoflagellate Oxyrrhis marina. Sprecher BN, Zhang H, Lin S. Microorganisms. 2020 Jan 16;8(1). pii: microorganisms8010126. doi: 10.3390/microorganisms8010126. 10.3390/microorganisms8010126 PubMed 31963386
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