DinoIII-arrO
(Plasmid
#128142)
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PurposeDinoflagellate backbone for rifampin resistant codon optimized gene
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 128142 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMD19 T-Vector
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Backbone manufacturerTakara
- Backbone size w/o insert (bp) 2679
- Total vector size (bp) 5590
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Vector typeBacterial Expression
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Selectable markersRifampin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRifampin ADP-ribosylating transferase
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Alt namearrO
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SpeciesCodon optimized for Oxyrrhis marina
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Insert Size (bp)453
- Promoter Dinolagellate potential promoters
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (destroyed during cloning)
- 3′ cloning site BglII (destroyed during cloning)
- 5′ sequencing primer M13R
- 3′ sequencing primer M13F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
DinoIII-arrO was a gift from Senjie Lin (Addgene plasmid # 128142 ; http://n2t.net/addgene:128142 ; RRID:Addgene_128142) -
For your References section:
Nuclear Gene Transformation in the Dinoflagellate Oxyrrhis marina. Sprecher BN, Zhang H, Lin S. Microorganisms. 2020 Jan 16;8(1). pii: microorganisms8010126. doi: 10.3390/microorganisms8010126. 10.3390/microorganisms8010126 PubMed 31963386
Map uploaded by the depositor.
