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pBEAST_J23101-amidase
(Plasmid #128135)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128135 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBEAST
  • Backbone size w/o insert (bp) 2987
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    amidase
  • Alt name
    Benzamid amidase (EC: 3.5.1.4), UniProtKB - B4XEY3
  • Species
    From Rhodococcus erythropolis, codon optimized for E. coli
  • Promoter J23101

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI, golden gate (unknown if destroyed)
  • 3′ cloning site BsaI, golden gate (unknown if destroyed)
  • 5′ sequencing primer ggcaactgaaacgattcg
  • 3′ sequencing primer ttgatgcctggcaccaac
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBEAST_J23101-amidase was a gift from Jean-Loup Faulon (Addgene plasmid # 128135 ; http://n2t.net/addgene:128135 ; RRID:Addgene_128135)

  • For your References section:

    Metabolic perceptrons for neural computing in biological systems. Pandi A, Koch M, Voyvodic PL, Soudier P, Bonnet J, Kushwaha M, Faulon JL. Nat Commun. 2019 Aug 28;10(1):3880. doi: 10.1038/s41467-019-11889-0. 10.1038/s41467-019-11889-0 PubMed 31462649
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